Sample result 2
Amino Acid Analysis Facility
SICKKIDS ADVANCED PROTEIN TECHNOLOGY CENTRE
Dept. of Structural Biology & Biochemistry
The Hospital for Sick Children 1 of 1
DATE:_____________
Sample Information
Vial: 5 Injection: 1 Dil_Vol: (uL) 60 Injection Volume: 15 ul Date Acquired
Sample File Name:
Acq Method Set Hydrolyzates 3B1A Method Set
Investigator:
Date Processed
Processing Method Hydrolyzates 3B1Nleu
Calibration Standard
Pierce Amino Acid Standard H; Derivatized with PITC; Pico-Tag column (3.9 mm X 15 cm); Column Temperature: 38 C; Waters Modified Pico-Tag Gradient; Waters Alliance 2690 Separations Module; Waters 24B7 Dual Wavelength Absorbance Detector; Detection at 254 nm; Waters Millennium 32 Chromatography Software
| Name | RT | Area | Amount |
|---|---|---|---|---|
1 | Asp | 1.591 | 25679 | 137.540 |
2 | Glu | 1.713 | 421318 | 2281.884 |
3 | Ser | 2.910 | 70541 | 391.859 |
4 | Gly | 3.138 | 2796510 | 15463.197 |
5 | Thr | 4.021 | 23624 | 129.248 |
6 | Ala | 4.192 | 3819360 | 19941.699 |
7 | Pro | 4.406 | 1582858 | 7959.346 |
8 | NH3 | 4.712 | 120234 | 1180.729 |
9 | Tyr | 6.641 | 135482 | 658.973 |
10 | Val | 7.405 | 2354586 | 11597.781 |
11 | Ile | 9.494 | 269875 | 1292.841 |
12 | Leu | 9.723 | 255269 | 1268.381 |
13 | Nieu | 10.134 | 2100363 | 10643.300 |
14 | RgntPK1 | 10.345 | 18021 | 441.372 |
15 | RgntPK2 | 10.565 | 533940 | 13476.126 |
16 | Phe | 10.826 | 271322 | 1313.307 |
17 | Lys | 12.000 | 668475 | 1744.626 |