Equipment
Applied Biosystems/MDS Sciex API QSTAR XL Pulsar coupled with a Agilent nano HPLC 1100 series
- Quantitation using iCAT or iTRAQ
- Accurate mass determination of peptides and intact proteins up to 80 kDa (using infusion-ESI)
- Accurate mass determination of small molecules (using infusion-ESI)
- Phosphorylation site determination
- De novo sequencing
Applied Biosystems/MDS Sciex API QSTAR XL Pulsar MALDI QTOF
- Accurate mass determination of peptides and intact proteins up to 35 kDa
- Peptide sequencing
- Phosphorylation site determination
- Protein identification – peptide map fingerprinting and MS/MS
- De novo sequencing
ThermoElectron LCQ Deca XP coupled with an Agilent capillary HPLC 1100 series
- Protein identification of gel and ‘gel-less’ proteins
- identification of Post-Translational Modifications
- MUDPit analysis (2D LC)
LC Packings Ultimate 2D LC system with a Probot MALDI spotter
- Off-line two-dimensional separation via strong-cation exchange (SCX) and reverse phase (RP) chromatography of complex biological samples. Fractions are collected as spots on a MALDI target plate.